Abacwaningi basebenzise ipayipi elisha lokuhlola isotopu elisezingeni eliphezulu (HT-SIP) kanye ne-metagenomics ukuze bathole ukubuka kokuqala kwe-microbiome esebenzayo ezungeze isitshalo esizuzisayo se-symbiont, i-arbuscular mycorrhizal fungus (AMF). Ikhredithi: Lawrence Livermore National Laboratory
Ukuxhumanisa ukuthi angobani amagciwane asendle nezici zawo zomzimba kanye nemisebenzi yezemvelo kuyinhloso eyinhloko yezazi zesayensi yezinto eziphilayo zemvelo. Kumasu alwela lo mgomo, I-Stable Isotope Probing—SIP—ibhekwa njengephumelela kakhulu ukutadisha ama-microorganisms asebenzayo ezindaweni zemvelo.
Ososayensi bakwa-Lawrence Livermore National Laboratory (LLNL) basungule indlela entsha—i-SIP ephezulu—ezenza ngokuzenzakalelayo izinyathelo ezimbalwa ohlelweni lokuhlola isotopu oluzinzile, okuvumela ukuphenywa komsebenzi we-microbial of microorganisms ngaphansi kwezimo ezingokoqobo, ngaphandle kwesidingo sokukhuliswa kwelebhu.
Ku-SIP, ama-microbes asebenzayo akhonjwa ngokufakwa kwama-isotopes azinzile ku-biomass yawo. Iphakathi kwezindlela ezinamandla kakhulu ku-microbial ecology njengoba ikwazi ukubona amagciwane asebenzayo kanye nezici zabo zomzimba (ukusetshenziswa kwe-substrate, i-biochemistry yamaselula, i-metabolism, ukukhula, ukufa) emiphakathini eyinkimbinkimbi ngaphansi kwezimo zendawo.
Ngokuvamile, indlela ye-SIP idinga ukusebenza kanzima futhi ivumela kuphela inani elincane lamasampuli. Kodwa indlela entsha ye-LLNL idinga ingxenye eyodwa kwesithupha yenani lomsebenzi owenziwa ngezandla uma kuqhathaniswa ne-SIP yemanuwali futhi ivumela amasampula ayi-16 ukuthi acutshungulwe kanyekanye.
"Indlela yethu ye-semi-automated yehlisa isikhathi somsebenzisi futhi ithuthukisa ukuzala kabusha ngokukhomba izinyathelo ezidinga abasebenzi abaningi ze-SIP," kusho usosayensi we-LLNL u-Erin Nuccio, kanye nombhali oholayo wephepha elivela kumagazini iMicrobiome. "Manje sesisebenzise le ndlela ukucubungula amasampula angaphezu kwenkulungwane, okuhlanganisa namanye avela ezindaweni ezincane ezingafundile."
Enye i-microhabitat enjalo inhlabathi ezungeze ngokushesha izicubu ze-mycorrhizae—uhlobo lwesikhunta olwakha ubudlelwano be-symbiotic ne-72% yazo zonke izitshalo zasezweni. Ngokushintshanisa i-carbon yezitshalo, ukhunta (isikhunta se-arbuscular mycorrhizal) sinikeza abasingathi baso izinsiza ezibalulekile njenge-nitrogen, i-phosphorus namanzi.
Kulolu cwaningo lobufakazi bomqondo, ababhali babonise "iwebhu yokudla" yokusebenzelana okugqugquzelwa isikhunta se-mycorrhizal enhlabathini.
“Sicabanga ukuthi lena yindlela enkulu yokuthi ikhabhoni yezitshalo isatshalaliswe kabanzi enhlabathini. I-Soil iphethe ichibi elikhulu kunawo wonke lokushisekela i-organic carbon emhlabeni,” kusho umbhali ohambisana naye u-Jennifer Pett-Ridge, ohola iphrojekthi ye-LLNL nenhloko yeHhovisi Lomnyango Wezamandla WezeSayensi “I-Microbes Persist” Soil Microbiome Scientific Focus Area. . "Silandele inani elincane le-DNA, sanquma izinto eziphilayo ezisebenzayo bese sakha kabusha ama-genomes kanye nokusebenzisana okungenzeka."
Abanye ababhali be-LLNL bahlanganisa uSteven Blazewicz, uMarissa Lafler, u-Ashley Campbell, uJeffrey Kimbrel, uJessica Wollard, uRachel Hestrin kanye nabacwaningi abavela kuLawrence Berkeley National Laboratory, i-DOE Joint Genome Institute kanye neNyuvesi yaseCalifornia, eBerkeley.